Zinc Detoxification Is Required for Full Virulence and Modification of the Host Leaf Ionome by Xylella fastidiosa.
Identifieur interne : 000421 ( Main/Exploration ); précédent : 000420; suivant : 000422Zinc Detoxification Is Required for Full Virulence and Modification of the Host Leaf Ionome by Xylella fastidiosa.
Auteurs : Fernando Navarrete [États-Unis] ; Leonardo De La FuenteSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2015.
Descripteurs français
- KwdFr :
- Feuilles de plante (composition chimique), Feuilles de plante (microbiologie), Ions (analyse), Maladies des plantes (microbiologie), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Techniques de knock-out de gènes (MeSH), Virulence (génétique), Virulence (physiologie), Vitis (microbiologie), Xylella (composition chimique), Xylella (métabolisme), Xylella (pathogénicité), Zinc (métabolisme).
- MESH :
- analyse : Ions.
- composition chimique : Feuilles de plante, Xylella.
- génétique : Protéines bactériennes, Virulence.
- microbiologie : Feuilles de plante, Maladies des plantes, Vitis.
- métabolisme : Protéines bactériennes, Xylella, Zinc.
- pathogénicité : Xylella.
- physiologie : Virulence.
- Techniques de knock-out de gènes.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Gene Knockout Techniques (MeSH), Ions (analysis), Plant Diseases (microbiology), Plant Leaves (chemistry), Plant Leaves (microbiology), Virulence (genetics), Virulence (physiology), Vitis (microbiology), Xylella (chemistry), Xylella (metabolism), Xylella (pathogenicity), Zinc (metabolism).
- MESH :
- chemical , analysis : Ions.
- chemical , genetics : Bacterial Proteins.
- chemical , metabolism : Bacterial Proteins, Zinc.
- chemistry : Plant Leaves, Xylella.
- genetics : Virulence.
- metabolism : Xylella.
- microbiology : Plant Diseases, Plant Leaves, Vitis.
- pathogenicity : Xylella.
- physiology : Virulence.
- Gene Knockout Techniques.
Abstract
Zinc (Zn) is an essential element for all forms of life because it is a structural or catalytic cofactor of many proteins, but it can have toxic effects at high concentrations; thus, microorganisms must tightly regulate its levels. Here, we evaluated the role of Zn homeostasis proteins in the virulence of the xylem-limited bacterium Xylella fastidiosa, causal agent of Pierce's disease of grapevine, among other diseases. Two mutants of X. fastidiosa 'Temecula' affected in genes which regulate Zn homeostasis (zur) and Zn detoxification (czcD) were constructed. Both knockouts showed increased sensitivity to Zn at physiologically relevant concentrations and increased intracellular accumulation of this metal compared with the wild type. Increased Zn sensitivity was correlated with decreased growth in grapevine xylem sap, reduced twitching motility, and downregulation of exopolysaccharide biosynthetic genes. Tobacco plants inoculated with either knockout mutant showed reduced foliar symptoms and a much reduced (czcD) or absent (zur) modification of the leaf ionome (i.e., the mineral nutrient and trace element composition), as well as reduced bacterial populations. The results show that detoxification of Zn is crucial for the virulence of X. fastidiosa and verifies our previous findings that modification of the host leaf ionome correlates with bacterial virulence.
DOI: 10.1094/MPMI-07-14-0221-R
PubMed: 25561271
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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element for all forms of life because it is a structural or catalytic cofactor of many proteins, but it can have toxic effects at high concentrations; thus, microorganisms must tightly regulate its levels. Here, we evaluated the role of Zn homeostasis proteins in the virulence of the xylem-limited bacterium Xylella fastidiosa, causal agent of Pierce's disease of grapevine, among other diseases. Two mutants of X. fastidiosa 'Temecula' affected in genes which regulate Zn homeostasis (zur) and Zn detoxification (czcD) were constructed. Both knockouts showed increased sensitivity to Zn at physiologically relevant concentrations and increased intracellular accumulation of this metal compared with the wild type. Increased Zn sensitivity was correlated with decreased growth in grapevine xylem sap, reduced twitching motility, and downregulation of exopolysaccharide biosynthetic genes. Tobacco plants inoculated with either knockout mutant showed reduced foliar symptoms and a much reduced (czcD) or absent (zur) modification of the leaf ionome (i.e., the mineral nutrient and trace element composition), as well as reduced bacterial populations. The results show that detoxification of Zn is crucial for the virulence of X. fastidiosa and verifies our previous findings that modification of the host leaf ionome correlates with bacterial virulence. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25561271</PMID><DateCompleted><Year>2015</Year><Month>11</Month><Day>06</Day></DateCompleted><DateRevised><Year>2015</Year><Month>04</Month><Day>11</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>28</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Zinc Detoxification Is Required for Full Virulence and Modification of the Host Leaf Ionome by Xylella fastidiosa.</ArticleTitle><Pagination><MedlinePgn>497-507</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-07-14-0221-R</ELocationID><Abstract><AbstractText>Zinc (Zn) is an essential element for all forms of life because it is a structural or catalytic cofactor of many proteins, but it can have toxic effects at high concentrations; thus, microorganisms must tightly regulate its levels. 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